GEメーカーRPN2510Eの使用説明書/サービス説明書
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GE Healthcare Amersham Hybridization oven/shak er Pr oduct User Manual Codes: RPN2510E RPN2511E RPN2512E.
RPN2510EUM Pagefinder Rev C 06/2007 2 Page finder 1. Legal 3 2. Introduction 4 3. Safety warnings and precautions 5 3.1. Electrical Installation 5 4. Specification 6 5. Setting up the Hybridization O ven/Shak er 7 5.1. Setting the oven temperature 7 5.
1. Legal GE, imagination at work and GE monogram are trademarks of General Electric Company. Amersham, Alkphos Direct , ECF , ECL , ECL Plus, ECL Advance, Hybond, Hypercassette, Hyperfilm, Hyperscreen, Hyper torch, Megaprime, Nick, Rapid- hyb, Ready-To-Go , Rediprime, Sensitive and Trackertape are trademarks of GE Healthcare companies.
2. Intr oduction One of the most widely used techniques in the molecular biolog y field is the immobilization of DNA and RNA onto a solid suppor t membrane and subsequent hybridizations with a specific single stranded probe, labeled to facilitate its detection.
3. Safety w arnings and pr ecautions If the equipment is not used in the manner described in this manual the protection provided might be impaired This equipment is designed to operate under the follo.
4. Specification Overall dimensions Height: 17” (43.5 cm) Depth: 15” (38.0 cm) Width: 15” (38.0 cm) Oven dimensions Height: 8” (20.0 cm) Depth: 9” (23.0 cm) Width: 10” (25.0 cm) Weight: 24 kg Capacity (nominal): 18 litres T emperature range: Ambient plus 5°C–80°C T emperature precision: +/- 0.
5. Setting up the Hybridization O ven/Shaker RPN2510EUM Chapter 5 Rev C 06/2007 7 Fig 1. Diagram of instrument control panel Rotisserie/shaker controls 1. Rotisserie/shaker ON/OFF button 2. Rotisserie/shaker speed selector dial 3. Motor on indicator Temperatur e controls 4.
4. Place the rotisserie into the oven onto the rotation mechanism, ensuring that the serrated bands at either end of the rotisserie locate onto the steel cogs of the rotation mechanism at the rear of the oven. (B on figure 2). The plastic flanges of the r otisserie locate on to the small wheels on the oven floor .
RPN2510EUM Chapter 6 Rev C 06/2007 9 6. Hybridization using the platform shaker The Hybridization Oven/Shaker is compatible with the hybridization technologies available from GE Healthcare.
RPN2510EUM Chapter 7 Rev C 06/2007 10 Several advantages are associated with performing hybridizations in bottles, namely those of safety and economy as outlined in the introduction. However , the use of bottles for hybridizations and washing procedures requires certain adaptations to standard protocols.
RPN2510EUM Chapter 7 Rev C 06/2007 11 4. Prehybridize the membrane for the specified length of time at a rotisserie speed of 4 rpm. 5. Following prehybridization, turn off the rotisserie, remove the rotisserie from the oven.
RPN2510EUM Chapter 8 Rev C 06/2007 12 8. Maintenance/car e/cleaning of the oven/shak er The GE Healthcare Hybridization Oven/Shaker is designed to provide trouble-free operation. The base of the oven and the shaker tray act as a spills tray and will contain any spillage that occurs during hybridization and washing procedur es.
RPN2510EUM Chapter 9 Rev C 06/2007 13 Symptom 1. Membrane curling up in hybridization bottle 2. High background 3. Weak signal 4. Pat chy backgrounds 5. High background around edge of membrane Cause 1.1. Incorrect orientation of bottle in rotisserie 2.
Appendix 1. Pr oducts for electr ophor esis DNA Markers Precise Sizing Digest of Natural DNAs 50 Base-pair 100 Base-pair KiloBase DRigest III ladder ladder DNA marker 27-4060-01 27-4005-01 27-4007-01 .
Appendix 2. Hybond membranes for nucleic acid applications Size Pack size Hybond N+ Hybond XL Hybond N Hybond NX Hybond ECL Hybond C Extra Hybond P 82 mm 50 discs RPN82B RPN82S RPN82N RPN82T RPN82D RP.
Appendix 3. Radioactive labeling systems Labelling T echnology Nucleotide Amount of Labelling Probe specific Recommended Product system template time activity application code (dpm/µg) Rediprime ™ .
Appendix 4. Non-radioactive labeling and detection systems Labelling and detection Sensitivity Time from Duration of Strip before Quantification Recommended application system hybridization light output re-pr obing to detection AlkPhos Direct (RPN3690) 0.
Appendix 5. Pr oducts for autoradiography and chemiluminscent detection Hyperfilm – high performance autoradiography films Product description size number sheets code Hyperfilm ECL 5 × 7 inches 50 .
Appendix 6. Hybridization buffer Product name Pack size Code Rapid-Hyb buffer Rate enhanced hybridization buffer 125 ml RPN1635 for use with radiolabelled nucleic acid probes 500 m RPN1636 RPN2510EUM .
Appendix 7: Radiation safety pr oducts Product Description Product Dimensions Quantity Code Number CDC storage box 240 × 140 × 130 mm 1 box RPN2032 Pipette guards Beta pipette guard, Gilson P20/100 .
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RPN2510EUM Rev C 06/2007 imagination at work GE Healthcare offices: GE Healthcare Bio-Sciences AB Björkgatan 30, 751 84 Uppsala, Sweden GE Healthcare Europe GmbH Munzinger Strasse 5, D-79111 Freibur g, Germany GE Healthcare Bio-Sciences Corp. 800 Centennial Avenue, P .
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GE RPN2510Eをまだ購入していないなら、この製品の基本情報を理解する良い機会です。まずは上にある説明書の最初のページをご覧ください。そこにはGE RPN2510Eの技術情報の概要が記載されているはずです。デバイスがあなたのニーズを満たすかどうかは、ここで確認しましょう。GE RPN2510Eの取扱説明書の次のページをよく読むことにより、製品の全機能やその取り扱いに関する情報を知ることができます。GE RPN2510Eで得られた情報は、きっとあなたの購入の決断を手助けしてくれることでしょう。
GE RPN2510Eを既にお持ちだが、まだ読んでいない場合は、上記の理由によりそれを行うべきです。そうすることにより機能を適切に使用しているか、又はGE RPN2510Eの不適切な取り扱いによりその寿命を短くする危険を犯していないかどうかを知ることができます。
ですが、ユーザガイドが果たす重要な役割の一つは、GE RPN2510Eに関する問題の解決を支援することです。そこにはほとんどの場合、トラブルシューティング、すなわちGE RPN2510Eデバイスで最もよく起こりうる故障・不良とそれらの対処法についてのアドバイスを見つけることができるはずです。たとえ問題を解決できなかった場合でも、説明書にはカスタマー・サービスセンター又は最寄りのサービスセンターへの問い合わせ先等、次の対処法についての指示があるはずです。